![]() Low resolution in identification of closely-spaced multimodal size distributions.It is limited to offline and at-line methods of use.The following limitations apply to DLS and direct imagining particle size analysers: With any analytical method, there exist several difficulties and limitations. This allows Eyecon 2 to more accurately calculate particle volume, which will affect the subsequently calculated D-Values. ![]() The cubed diameter of an equivalent circle fit used in many other image analysis systems. This approach results in a tighter volume fit than EyePASS uses a particle detection algorithm to identify and measure individual particles within the captured image. The application of the Mie theory can then be used to determine approximate particle volume and several other parameters.Įyecon 2 identifies particles within a sample image which are then processed by EyePASS. The initial step of DLS is extracting the particle information and monitoring the change in the scattered intensity over time at a high temporal resolution, on the order of nano to a millisecond, and determine the correlation of the changing signal to The images captured are processed by the software EyePASS™ where the particle detection algorithm identifiesĪnd measures individual particles within the image. Our in-house advanced particle size analyser, Eyecon 2,Ĭalculates particle size distributions, and shape information based on the measurement of individual particles within a sample image. Where various areas of bright and dark spots represent the constructive (bright spots) and destructive (dark spots) regions. If this process were to be imaged, a speckle pattern would be observed, Therefore, given the large light intensity associated with DLS setups, many photons can interfere both constructively and destructively due to the many possible scattering angles. When the scattered waves interference is constructive, the DLS detector records high light intensity and equally, when waves interference is destructive, the detector records low light intensity.įigure: Constructive and Destructive Interference Waves, as is presented in the figure below. When two waves interact, they can combine in such a manner that the resultant wave is either an amplified or diminished superposition of the initially interacting The fundamental principle governing the determination of particle size by DLS is interference. We have selected cutting-edge direct imaging particle size analysers, such as Eyecon 2, to explain the fundamentals of image analysis which will give you an appreciation of some of the factors to consider in your choice of particleĭLS, sometimes referred to as Quasi-Elastic Light Scattering (QELS) and Photon Correlation Spectroscopy (PCS), is an optical method of determining particle size properties within a disperse sample by observing the change in scattered light intensity asĪ function of time. We have created a detailed review of industry-standard methods such as Dynamic Light Scattering (DLS) and image analysis, where we contrast the principles of DLSĪgainst image analysis. ![]() Exploring the particle size measurement landscape can be challenging and time-consuming. ![]()
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